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GPOs are suddenly not being applied on an RDS server

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We just noticed yesterday afternoon that policies for an RDS server (Windows 2008 R2) that is hosted in the cloud are not being applied. There are two domain controllers also Windows 2008 R2.  When I run an RSOP on a DC, the information returned looks OK, but some obvious settings are not applied at the RDS server. Also, for existing users, environmental variables are fine, but for new users, they are incorrect. The RDS server was updated and rebooted last weekend, so it's possible that some updates have caused the problem. I'm not sure what to try next, so does anyone have any troubleshooting techniques I might try?

There are several of the following log entries (Event ID 1530, User Profile Service):

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Windows detected your registry file is still in use by other applications or services. The file will be unloaded now. The applications or services that hold your registry file may not function properly afterwards.  

 DETAIL - 
 1 user registry handles leaked from \Registry\User\S-1-5-21-3322582763-3368561368-2352044815-595106:
Process 12 (\Device\HarddiskVolume2\Windows\System32\svchost.exe) has opened key \REGISTRY\USER\S-1-5-21-3322582763-3368561368-2352044815-595106\Printers\DevModePerUser

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There were also several of the following event IDs 4625:

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An account failed to log on.

Subject:
Security ID: SYSTEM
Account Name: RDSSERVER$
Account Domain:RDSDOMAIN
Logon ID: 0x3e7

Logon Type:10

Account For Which Logon Failed:
Security ID: NULL SID
Account Name: admin
Account Domain:RDSDOMAIN

Failure Information:
Failure Reason:Unknown user name or bad password.
Status: 0xc000006d
Sub Status: 0xc0000064

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I reset the machine account using Reset-ComputerMachinePassword, and I no longer see these errors, but the problem is still not fixed. I'd sure appreciate any thoughts on this.


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